This article investigates the influence of different nanofluid distributions on the treatment temperature by considering a homogeneous distribution of magnetic nanoparticles (MNPs). Two distributions are observed in experimental images and modeled by the proposed method in this article. Meanwhile, the effect of nanofluid diffusion on the concentration distribution is further studied. In addition, the difference of temperature-based on the blood perfusion rate and the traditional quantitative perfusion rate is also considered. The treatment effect in this study is evaluated by the cumulative equivalent minutes at 43℃ ( CEM43) from the perspective of the thermal iso-effect dose, which is directly related to the treatment temperature distribution. Simulation results demonstrate that the nanofluid diffusion has positive effect on the uniformity degree of nanofluid distribution inside tumor region, while it has less effect on the ultimate treatment temperature if individual critical power dissipations of MNPs.