To solve the problem of total focusing imaging of double-layer media under blind measurement conditions, this article proposes a total focusing method ( TFM) based on virtual source ( VS). Firstly, a series of virtual sources are established on the irregular interface of the double-layer media by using the first echo time-of-flight of the diagonal signals of full matrix capture data. Then, the irregular interface of the double-layer media is reconstructed by interpolating or fitting the virtual sources on the interface. Finally, the reconstructed interface is used for possible refraction points to achieve the TFM imaging condition for the irregular interface of doublelayer media. The results show that the position of the surface and flaws are both located correctly in TFM images under blind measurement conditions. Compared with the synthetic aperture focusing technique ( SAFT) imaging technology based on virtual source (VS), the signal-to-noise ratio of the VS-TFM images is improved by 4. 66~ 13. 31 dB in the convex specimen, and by 4. 74~ 12. 8 dB in the concave specimen, with nearly the same API values and minimal increase in imaging time. Therefore, the proposed method extends TFM imaging to double-layer media with irregular surfaces in blind measurement conditions.