The world′s first space optical clock, launched into orbit in 2022, has begun its experimental operations in space. The unique physical properties and complex structure of the space optical clock have resulted in significant differences between the ground calibration phase and the in-orbit operational phase. This study analyzes the variations in gravitational fields, temperature fields, and magnetic fields between Earth and space from both physical and technical perspectives, leading to the development of an assessment method for the gravitational redshift frequency shift of the space optical clock. Furthermore, we propose a technical solution for narrow-band atomic trapping in microgravity environments and discuss the impact of the spatial temperature and magnetic field environments on the clock′s performance. In ground experiments, the gravitational direction experienced by the atoms is altered by 180° via the rotation of the physical vacuum chamber. Subsequently, a compensatory Helmholtz magnetic field is introduced and finely adjusted, based on the existing anti-Helmholtz magnetic field of the magneto-optical trap, allowing for precise movement of the atom cloud′s center position within the secondary magneto-optical trap. When the compensatory magnetic field reaches 120 μT, the fluorescence intensity of the secondary atoms is restored to the state observed prior to the reversal of the gravitational direction, thereby validating the technology for atom position movement in the in-orbit red magnetic optical trap. We analyze the temperature data from the scientific cavity in orbit and fit the data using an exponential function, yielding a temperature-time index of approximately 138(7) minutes for the scientific cavity in orbit. This finding provides a foundational basis for future assessments of blackbody radiation frequency shifts of the space optical clock. Additionally, we test the shielding coefficient of the magnetic shield and, through theoretical analysis, establish that the shield can attenuate external magnetic fields by approximately 120 times, with its design and fabrication satisfying the operational requirements for in-orbit experiments of the space optical clock. Through the theoretical analysis and experimental validation presented in this article, we not only provide data and technical support for future in-orbit experiments of space optical clocks but also offer significant references for the design of next-generation space optical clocks.