Due to the impact of high-speed airflow impact under complex operating conditions in the wind tunnel, the wing produces dynamic bending and twisting deformation. This article proposes a fast measurement method that uses a single-double mixed stereo vision method, which can achieve the bending and twisting deformation measurement of the wing in the wind tunnel by using fluorescent markers. Firstly, the layout and computing model of the wing′s bending and twisting deformation is proposed. Then, a camera coordinate system is established to calibrate cameras based on the relative relationship of the wind tunnel coordinate system and the aircraft model′ s own coordinate system. Finally, a single camera 3D reconstruction model is constructed according to the wing points with known Y-axial constraints. Therefore, the dynamic bending and twisting deformation can be calculated under blowing condition. This method can realize the fast dynamic measurement of the wing bending and twisting deformation by establishing a special layout of dynamic wing deformation system. Based on the initial position of the aircraft model, the average error of the obtained twisting angle is 0. 228 °. It can effectively solve the problems of reconstruction with high frame rate in binocular cameras and provide data support for optimizing the design parameters in aircraft.