This paper presents the design and development of a crossaxis chromatograph. Firstly, the suitable mechanical structure is designed considering design requirements. The kinematics analysis is carried out to obtain the driving torque by the theorem of momentum moment. Secondly, the modal analysis is carried out in order to obtain the weak link of the structure. Finally, the structure of weak link is optimized to develop the prototype. The separation efficiency and influencing factors of the prototype are tested by experiments. The results show that, with the increase of the rotational speed in a certain range, the retention rate of stationary phase of the prototype increases, and the separation efficiency also increases. When the speed of the prototype reaches 800 r/min, the retention rate of stationary phase is the largest. The retention rate of stationary phase decreases when the speed exceeds 800 r/min, then the separation efficiency decreases. When the speed of the chromatograph keeps constant, the velocity of liquid in the chromatograph is greater and the retention rate of stationary phase is lower.