基于碳点的小型自校正比率荧光光纤铬(VI)传感器
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TH744

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河北省自然科学基金(F2020501040)、河北省高等学校青年拔尖人才计划(BJK2022069)、中央高校基本科研业务费(N2223035)项目资助


The miniature self-correcting ratiometric fluorescence optical fiber sensor based on carbon dots for chromium (VI) detection
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    摘要:

    荧光比率传感探针检测方法能够克服环境(如样品基质、猝灭剂等)和仪器等外部因素影响, 得到更加可靠的检测结 果。 本文合成用于组成比率型荧光纳米探针的两种荧光碳点(CDs), 在 365 nm 紫外光的激发下, 这两种碳点分别在 440 nm 和 570 nm 处具有荧光发射峰, 且对 Cr(VI)具有相反的响应。 通过水凝胶将比率荧光探针包覆在设计的光纤尖端上, 能够实 现 Cr(VI)浓度的在线检测。 利用飞秒激光微加工系统制作的齿形光纤结构, 有利于荧光探针的激发和传感器荧光的收集。 为进一步降低传感器成本、缩减传感器体积, 采用低成本的 LED 灯珠作为激发光源。 采用比率荧光探针包覆, 克服 LED 灯珠 光源稳定性差的缺点, 使传感器可以实现自校正。 实验表明,即使光源强度波动较大, 该传感器连续 7 次测量的相对标准偏差 (RSD)仅为 3. 1% 。 在 0~ 200 μM 范围内, 该传感器对 Cr(VI)具有良好的线性关系, 检出限(LOD)为 0. 9 μM。 该传感器能够 应用于实际样品中 Cr(VI)的检测, 说明该传感器的具有实用性和可靠性。

    Abstract:

    The influence of external factors from the environment (such as sample matrix, quenching agents, etc. ) and instruments can be reduced by the employment of ratiometric fluorescent probes to obtain more reliable detection results. In this article, two kinds of carbon dots (CDs) are synthesized and used as ratiometric fluorescent nanoprobes, which emit fluorescence at 440 nm and 570 nm, respectively, with 365 nm UV light excitation. The two kinds of CDs have opposite fluorescent responses to Cr (VI). The fluorescent probes are coated on the tip of the designed fiber tip with hydrogel to accomplish the online Cr ( VI) detection. The tooth-shaped structure on the fiber tip, which is processed by a femtosecond laser micromachining system, is beneficial to the excitation of fluorescent probes and the fluorescence collection of the sensor. A low-cost LED lamp is utilized as the excitation light source to save the cost and reduce the size of the sensor. The disadvantage of poor stability of the LED light is overcome due to the use of ratiometric fluorescent probes. In consequence, the miniature sensor realizes the self-correcting. It is proved that even though the intensity of the light source fluctuates greatly, the relative standard deviation (RSD) of 7 consecutive measurements is only 3. 1% . In the range of 0~ 200 μM, the sensor has a great linear relationship for Cr (VI) detection with a detection limit (LOD) of 0. 9 μM. The practicability and reliability of the sensor are demonstrated by the determination of Cr (VI) in real-world water samples.

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蔡 忆,姜天雨,张 静,李 意,赵 勇.基于碳点的小型自校正比率荧光光纤铬(VI)传感器[J].仪器仪表学报,2023,44(11):261-270

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  • 在线发布日期: 2024-01-29
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